Upon the properties of an antityphoid serum obtained from the goat / by Allan Macfadyen ; communicated by C.J. Martin.

  • Macfayden, Allan, 1860-1907.
Date:
1906
Catalogue details

Licence: In copyright

Credit: Upon the properties of an antityphoid serum obtained from the goat / by Allan Macfadyen ; communicated by C.J. Martin. Source: Wellcome Collection.

Provider: This material has been provided by The Royal College of Surgeons of England. The original may be consulted at The Royal College of Surgeons of England.

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    Conclusions. 1. The intravenous injection of the goat with the toxic cell juices of the B. typhosus (obtained under the conditions described) in small and carefully regulated doses resulted in the production of an antiendotoxin. 2. The antiendotoxic value, as so far tested, reached a point at which 1/50 c.c. of the serum neutralised 20 lethal doses of the toxic typhoid cell juice. This action was not demonstrable in 3 c.c. of normal goat’s serum, and was obtained after about four months’ treatment, of the goat. The results, after a more rapid method of immunisation, are better qua goat and rabbit than those obtained by Dr. Besredka in the course of two years with dead and living bacilli qua horse and guinea-pig. 3. The serum was also agglutinative for the B. typhosus, the titrate rising to 1/1,000,000. 4. The serum was also bacteriolytic, 1/10000 c.c. neutralising 10 lethal doses of the B. typhosus. 5. The serum did not give a precipitin reaction with typhoid cell juices. 0. The serum whilst neutralising the typhoid did not neutralise the cholera endotoxin. My next step will be to test in how far it is possible to obtain analogous results in the horse. Analogous results have been obtained indicating the production of an anti- body for the endotoxin of the cholera organism. I am greatly indebted to Mr. E. T. Thompson for invaluable aid as well as for an important modification which lias rendered the grinding process void of danger. Harbison AND Sons, Printers in Ordinary to His Majesty, St. Martin’s Lane.