Upon the properties of an antityphoid serum obtained from the goat / by Allan Macfadyen ; communicated by C.J. Martin.

  • Macfayden, Allan, 1860-1907.
Date:
1906
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Licence: In copyright

Credit: Upon the properties of an antityphoid serum obtained from the goat / by Allan Macfadyen ; communicated by C.J. Martin. Source: Wellcome Collection.

Provider: This material has been provided by The Royal College of Surgeons of England. The original may be consulted at The Royal College of Surgeons of England.

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    :\€?\ «, o no / \ \ u cj / * \ / Ctj / Upon the Properties of an Antityphoid Serum obtained from the '§_\ Goat. By Allan Macfadyen, M.D. (Communicated by Dr. C. J. Martin, F.R.S. Received March 2,—Read March 8, 1906.) (From the Bacteriological Department, Lister Institute.) In this communication I propose to give a brief account of the results obtained by the immunisation of the goat with the cell juices of the Bacillus typhosus, in continuation of researches already published which had for their main object the production of an antibody for the endotoxin of the typhoid organism.* These experiments met with partial success and failure. It will be sufficient on the present occasion to say that the goat proved a more suitable animal than the horse for arriving at a solution of the theoretical and practical considerations involved. The object was to arrive at the best method of producing with the material employed an antiendotoxin in adequate amount, with a view to its reapplication to the horse. During the progress of these researches experiments on analogous lines have been published by Dr. Besredka,t and to these I will, in the first instance, refer. Dr. Besredka describes the results following the intra- venous injection into a horse of the intact dead and living typhoid bacilli. The injections of the bacilli were carried out for a period of two years. The serum was tested for antiendotoxic properties against (1) killed and dried cultures of the B. typhosus, of which 0-01 gramme killed guinea pigs on intraperitoneal injection; (2) a soluble endotoxin extracted from the dead and dried bacilli, of which 1/8 c.c. killed guinea pigs. The results were as follows: 10 or 15 centigrammes of the dried serum (about 1 c.c. aud 1*5 c.c. before drying) neutralised 10 or 15 lethal doses of the killed typhoid culture respectively on intraperitoneal injection into the guinea pig, and the 10 centigrammes of the dried serum neutralised 16 lethal doses of the soluble * “Upon the Immunising Effects of the Intracellular Contents of the Typhoid Bacillus, as obtained by the Disintegration of the Organism at the Temperature of Liquid Air,” by Allan Macfadyen, ‘Roy. Soc. Proc.,’ March 12, 1903; “Upon the Intracellular Consti- tuents of the Typhoid Bacillus,” by Allan Macfadyen and Sydney Rowland, ‘ Centralblatt fur Bakteriologie,’ Abth. 1, vol. 30, 1901, No. 20; and vol. 34, 1903, Nos. 7 and 8. t “Etudes sur le bacille typhique et le bacille de Peste,” par Dr. Besredka, ‘ Annales de lTnstitut Pasteur,’ July 25, 1905: “De l’Antiendotoxine Typhique,” par Dr. Besredka, Ibid., February, 1906.