Studies in isoagglutination. III. Tonicity in isohemagglutination / by Morris H. Kahn and Reuben Ottenberg.

  • Kahn, Morris H.
Date:
[1911]
Catalogue details

Licence: In copyright

Credit: Studies in isoagglutination. III. Tonicity in isohemagglutination / by Morris H. Kahn and Reuben Ottenberg. Source: Wellcome Collection.

Provider: This material has been provided by The Royal College of Surgeons of England. The original may be consulted at The Royal College of Surgeons of England.

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    successive concentrations was 0.03 per cent., as in Gay s work. Later it was made 0.02 per cent. All precautions were taken to make the solutions accurate and constant. Chemically pure sodium chlorid dried to constant weight was used, and fresh solutions were prepared for each new group of cases tested. Evaporation was carefully prevented. In testing each blood for tonicity, a series of small test tubes was arranged, each tube containing five cubic centimeters of one of the graded salt solutions. The sedimented unwashed red cells were shaken, to insure uniform distribution, and 0.1 cubic centimeter of the suspension was added to each tube. The tubes were shaken and allowed to stand at room temperature for a short time. Read- ings were made then, and again after the tubes had been in an ice box for twelve hours. The amount of hemolysis was estimated for each tube of the series by comparing its color with the colors of a standard series made up separately with each blood. This scale was prepared by laking 0.4 cubic centimeter of the sedimented red cells in twenty cubic centimeters of distilled water (the same ratio of blood as that in the tests). This was designated 100 per cent, hemolysis and dilutions of this were then made to represent 80, 70, 6o_, 40, 30, 20, 10, 5, and 2.5 per cent, hemolysis. Each of these solutions was put in a tube of the same caliber as that of the tubes used in the tests. In order to facilitate our discussion, we reproduce Gay’s3 tables. Gay’s Results. Non-Agglutinable Cells. Class I. NaCl solution ( j!). | 0.39 | Q-42 | °-45 | °-48 [ Q-51 1 Amount of hemolysis (percentage). Blood No. 18 IOO 75 40 s 5 Aug. 26 100 IOO 60 35 10 Sept. 24 “ 22 IOO 90 75 50 2.5 Aug. 18 IOO 40 15 5 2.5 Aug. 26 •• 34 80 80 35 15 10 “ 35 95 50 15 10 5 38 95 35 10 10 2.5 Gay, Jour. Med. Research, 1907-8, xvii, 33°.