On neurokeratin / by W. Kühne ... and R.H. Chittenden.

  • Kühne, W. (Willy), 1837-1900.
Date:
1890
Catalogue details

Licence: Public Domain Mark

Credit: On neurokeratin / by W. Kühne ... and R.H. Chittenden. Source: Wellcome Collection.

Provider: This material has been provided by the Augustus C. Long Health Sciences Library at Columbia University and Columbia University Libraries/Information Services, through the Medical Heritage Library. The original may be consulted at the the Augustus C. Long Health Sciences Library at Columbia University and Columbia University.

    45/54 (page 41)
    they show a taadei (ten, then in dilute :.naJ- ly in water and in gljeerin, in the latter of which the \ aration can be best examined. In this manner all and rnmhrag are avoided and a preparaJ - .tied which, while in great part composed of twisted and k; - ?, still s - many pieces which may be ea-ily r. nized as residues >>t ner\e fibers, with the char joining of the outer and inner horny sheaths and ti rk of neurokeratin. In order te exclude all doul B he eompleten — the extraction of the myeliue bodies, the celloidin. which still surrouuds the residue from the nerve, can be complete- ly removed. This is best accomplished, after the final wash- _ with alcohol, by dropping upon the softened section placed on a h. ' _ tss slide boiling alcohol, and then the residue with ether, care being taken to avoid its :; ing away. Addition of 1 to-5-per-cent. car.- - will then show the character of the insoluble substauee. The best preparations are obviously obtained from regular 1. I _ tudinal sections of nerves, while trausverse and oblique - - lions are more apt to yield poorer and less satisfactory speci- mens. For mieroscopists we would on some accounts recom- mend the pancreatic instead of gastric digestion, in which the collagenous fibers take the place of celloidin in prevent- ing the eutire collapse of the nerve fiber in the after-treat- ment with alcohol, benzol, etc.. and in which, likewise, the volume and length of the fiber remain practically unaltered. Furthermore, mammalian nerves can remain in the warm trypsin solution as long aa desired, and that too without g ition of the mixture. The pieces can then be washed iu water, freed from myelitic bodies, spread upon appropri- I the fibers teased apart in the ordinary tray. One would hardly expect to rind, after this method of treat-