On neurokeratin / by W. Kühne ... and R.H. Chittenden.

  • Kühne, W. (Willy), 1837-1900.
Date:
1890
Catalogue details

Licence: Public Domain Mark

Credit: On neurokeratin / by W. Kühne ... and R.H. Chittenden. Source: Wellcome Collection.

Provider: This material has been provided by the Augustus C. Long Health Sciences Library at Columbia University and Columbia University Libraries/Information Services, through the Medical Heritage Library. The original may be consulted at the the Augustus C. Long Health Sciences Library at Columbia University and Columbia University.

    27/54 (page 23)
    cold and li<>t, and finally with alcohol and ether, after which it was dried on the tiller, first in t he air, t hen at 110° C, and weighed. These various determinations should convince those who do not now apparently believe in the coexistence within the nervous system of the myelitic bodies and of a substance resistanl to the action of the digestive fluids and of caustic alkalies that neurokeratin really does exist; for in the weighed residues from the nerve filters, in spite of all that they had passed through, we could plainly detect micro- scopically the hornv sheaths and the spongedike neuro- keratin in the peculiar knotty or gnarled structure of the fragments as well as in the separated short cylinders occa- sionally seen, in which might also be discovered a second still smaller cylinder of the inner horny sheath. The frag- ments were colored intensely blue by hematoxylin, while by Ileidenhain's coloring, with the supplementary action of chromates, they were stained a blackish blue. They likewise gave the lead reaction for sulphur. I. 25 grammes from both brachial plexites of a woman seventy-two years old gave 0079 gramme of neurokeratin = CK316 per cent. II. 50 grammes of the cortex of the cerebellum of a man twenty-one years old pave 0-1560 gramme of neurokeratin = 0-312 per cent. III. 50 grammes of white substance (not quite free from gray) from the cerebrum of the same man gave T1217 gramme of neuro- keratin = 2243 per cent. IV. 50 grammes of the pure white substance from the corpus callosum of a man fifty-seven years old gave 1'451 gramme of neurokera- tin = 2902 per cent. V. 50 grammes of the pray substance (as free ns possible from white) from the cortical portion of the cerebrum of the same man gave 01635 gramme of neurokeratin = 0327 per cent. The gray substance was separated from the white by scraping instead of by cutting, a method which enabled us to avoid any recognizable quantity of white matter.