An experimental inquiry into the nutrition of animal tissues / by William Marcet.

  • Marcet, W. (William), 1828-1900.
Date:
1874
Catalogue details

Licence: Public Domain Mark

Credit: An experimental inquiry into the nutrition of animal tissues / by William Marcet. Source: Wellcome Collection.

Provider: This material has been provided by The Royal College of Surgeons of England. The original may be consulted at The Royal College of Surgeons of England.

    17/56 (page 13)
    obtained. Thirty cubic centimetres of this fluid were used for the determination of albumen and of the nitrogen present in addition to that contained in the albumen; the operation was conducted as follows :— The Extract—Determination of Albumen.—The 30 c.c. of extract were mixed with as much or more water in a platinum capsule, and boiled. After boiling briskly for a few seconds the fluid Avas evaporated to dryness in a water bath, the residue was washed with hot distilled Avater, then hot alcohol, and finally a mixture of alcohol and ether, the washings being all run through the same filter. Finally, the albumen in the filter Avas washed back again into the platinum capsule with as little Avater as possible, and the whole albumen was dried, weighed, and incinerated, Avhen a very small proportion of ash was obtained; the difference between the weight of the capsule and its contents, before and after incineration, gave the weight of the albumen. This method yielded very satisfactory results, as will be seen by the fact that two equal quantities of the same extract of flesh yielded as near as possible the same weight of albumen. Tavo determinations of albumen in the same extract Avere frequently made in order to ensure the accu- racy of the result. I beg to subjoin the numbers obtained in some cases, taken, I might say at random, from my note- book, and which will show hoAV closely the results agreed: Albumen in 30 c.o. Extract Ox Flesh Ox Flesh Salmon Ox Lungs Ox Lungs Ox Lungs Human Tuber- cular Lungs Human Tuber- cular Lungs Human Tuber- cular Lungs Human Tuber- cular Lungs 1st deter- y 0-248 0-243 0-578 0-582 0-625 0-5585 0-338 0-310 0-2175 0-296 mi nation J 2nd dctor- y 0-250 0-239 0-570 0-586 0-632 0-558 0-342 0-310 0-2145 0-297 mination J Moan . . 0-249 0-241 0-574 0-584 0-629 0-558 0-340 0-310 0-206 0-296 Out of the ten analyses, the greatest difference between tAVO determinations of albumen in 30 c.c. of the same ex-