Upon the properties of an antityphoid serum obtained from the goat / by Allan Macfadyen, M.D. ; (communicated by Dr. C.J. Martin, F.R.S. Received March 2, - read March 8, 1906.).

  • Macfayden, Allan, 1860-1907
Date:
1906
Catalogue details

Licence: In copyright

Credit: Upon the properties of an antityphoid serum obtained from the goat / by Allan Macfadyen, M.D. ; (communicated by Dr. C.J. Martin, F.R.S. Received March 2, - read March 8, 1906.). Source: Wellcome Collection.

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    II. —Tests of Serum of Goat I. Date. Test animal. Amounts injected. Results. June 23. Rabbit 1 3 c.c. serum + 1 c.c. toxin. Alive. 33 . 33 2 Control, l c.c. toxin. Dead. 1 July 7 . 33 1 3 c.c. serum + 2 c.c. toxin. Alive. 33 . 33 2 3 „ +1 33 3 3 J) * * * *. 33 3 2 „ +1 >, 33 33 4 Control, 2 c.c. toxin. Dead, 4 hours. 33 . 33 5 „ 1/20 „ 33 18 „ July 14. 33 1 3 c.c. serum + 2 c.c. toxin. Alive. 33 . 33 2 2 „ +2 ,, 33 3' . 33 3 1 33 4 1 „ 33 33 . 33 4 1/10 „ +1 33 )) . 3 3 5 Control, 1/10 c.c. toxin. Dead. 33 ... 33 6 „ 1/20 „ Diarrhoea and collapse. July 21. 33 1 1 c.c. serum + 1 c.c. toxin. Alive. 33 . 33 2 1/2 „ +1 33 33 . 33 3 1/10 „ +1 „ 33 4 Control, 1 c.c. toxin. Dead. 33 . 33 5 „ 1 /10 Diarrhoea and collapse. 33 . 33 6 „ 1/16 33 33 33 August 4 . 33 1 1/20 c.c. serum+ 1 c.c. toxin. Alive. 33 .. 3» 2 1/50 „ +1 33 33 *•• . 33 3 1/100 „ +1 Control not made. 33 August 11. 33 1 1/20 c.c. serum + 1 c.c. toxin. 33 33 . 33 2 1/50 „ +1 „ 33 33 . 3 3 3 1/100 „ *1 „ Dead. 33 . 33 4 Control, 1 c.c. toxin. ,, 2 hours. 33 . 33 5 3/10 „ 3, 18 „ 33 . 33 6 „ i/io „ 33 18 ,3 The death of the goat prevented further injections and tests being made. It will be seen that demonstrable antiendotoxins had developed in the goat and that the results were encouraging. After 12 injections of typhoid cell juice, 1/50 c.c. of the goat’s serum protected a rabbit against 10 lethal doses of the typhoid endotoxin. This property was not present in 3 c.c. of normal goat’s serum. It is not my intention in this paper to refer in detail to the production of agglutinins and bacteriolysins. It will be sufficient to state that whilst the amount of agglutinins present in the serum varied, the highest titrate obtained was in a dilution of 1/1,000,000. After 12 injections of typhoid cell juice, 1/10000 c.c. of the goat’s serum protected guinea-pigs on intraperitonea 1 injection against 10 lethal doses of the B. typhosus. The serum was, therefore, proved to possess marked anti- endotoxic, agglutinative and bacteriolytic properties. The next step was to control these results by the immunisation of fresh