Studies on immunisation and their application to the diagnosis and treatment of bacterial infections / [Wright].

  • Wright, Almroth, 1861-1947.
Date:
1909
Catalogue details

Licence: In copyright

Credit: Studies on immunisation and their application to the diagnosis and treatment of bacterial infections / [Wright]. Source: Wellcome Collection.

Provider: This material has been provided by The University of Glasgow Library. The original may be consulted at The University of Glasgow Library.

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    volumes of blood, bacterial suspension and citrate of soda solution, by blowing these out upon a clean slide and re-aspirating several times in succession. Mixture completed, an aliquot portion of the mixed fluids, such as suffices for our purposes, is drawn up into the capillary stem, and the orifice of the capillary tube is sealed in the flame. This done, the pipette is placed either in an incubator standing at 37° C, or in a vessel of water kept at this temperature. After the lapse of fifteen minutes we break off the extremity of the pipette, carefully mix the contents so as to get an average sample, and proceed to make films, and then to stain them by Leishman's dye. Procedure No. 2, where we desire to elicit separately the role of the White Corpuscles and the Blood Fluids in Phagocytosis, and to study the Effect produced by Experimental Modification of one or other of these Elements separately. Having provided ourselves wdth a capsule with a recurved limb (such a capsule has already been figured in a previous communication),^ we introduce into it such a quantity of mercury as will fill it to about one- third of its capacity. Having marked off by a pencil mark (made wdth a glass writing pencil) the level at which the upper surface of the mercury stands, we displace the mercury in such a manner as to cause it to occupy the middle instead of the lower region of the capsule. We again mark off on the outside of the capsule the upper hmit of the mercury. Then, emptying out this last, we bend round in the flame the curved limb in such a manner as to cause it to lie in the plane of the equator of the capsule. This enables us to siphon into it from a watch glass, filled and placed ready to hand, the citrate of soda solution. We introduce of this solution such a quantity as suffices to fill the capsule up to the level of tlie first pencil mark. This done, we draw blood from the finger and let it run into the capsule until the combined volume of citrate of soda solution and blood attains the level of the second pencil mark. Having sealed up the upper orifice in the flame—^rarefying as we do so the air in the interior of the capsule by the apphcation of warmth— we shake up the contents and suspend the capsule by means of its curved hmb into the receptacle of the hand centrifugal machine. When centrifugahzation has caused the corpuscles to settle to the bottom, we pipette off and reserve the supernatant citrated plasma and replace it by physiological salt solution. In conducting this last operation we employ a capillary pipette, and we carry down its orifice to the very bottom of the capsule in such a manner as effectively to mix up the corpuscles and the newly added fluid. We wash and centrifugalize in this manner three times. The upper layers of the corpuscular deposit. '■ Wright, Roy. Soc. Proc, vol. Ixxi, 1902.