Acute pemphigus : a contribution to the aetiology of the acute bullous eruptions / by George Pernet and William Bulloch.

  • Pernet, George.
Date:
[1896?]
Catalogue details

Licence: Public Domain Mark

Credit: Acute pemphigus : a contribution to the aetiology of the acute bullous eruptions / by George Pernet and William Bulloch. Source: Wellcome Collection.

Provider: This material has been provided by The Royal College of Surgeons of England. The original may be consulted at The Royal College of Surgeons of England.

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    there was diffuse cloudiness, and by the end of twenty-four hours a deposit occurred on the bottom of the tubes, leaving the rest of the fluid comparatively clear. No membrane formed on the surface of the medium. On sloped agar the colonies at the end of six hours were in the form of small whitish specks. By the end of twenty-four hours they were of the size of a pin’s head. Further growth then took place by the shooting out of club-shaped processes from the periphery of the colony, so that in three to four days the whole colony had a rosette-like aspect, exactly as described and figured by Demme. On straight agar growth occurred along the whole track of the needle in the form of small lenticular-like colonies, while upon the free surface the growth resembled that on sloped agar. On blood-serum the colonies were less characteristic, the peripheral club-shaped expansions not being so clearly defined. On glycerinized potatoes at incubation temperature a glistening mother-of-pearl-like coating was formed on the surface. On gelatine at ordinary temperature a faint growth was observed after a week, but no marked liquefaction took place. Under conditions of anaerobiosis growths were obtained in liquid and solid media. On agar small whitish colonies were formed. Microscopic Examination.—The microscopic examination of the cultures, liquid and solid, showed that the micro-organism was a coccus, and occurred almost exclusively as a diplococcus, resembling the gonococcus, but somewhat larger. The two opposing surfaces were distinctly flattened, while the remaining surfaces were segments of a circle. The diplococci stained well by Gram’s method and its various modifications. They were also well stained by all hydro- alcoholic aniline stains. Experimental.—A guinea-pig, weighing 300 grammes, received 5 c.c. of the first bouillon culture under the skin, previously shaved and disinfected. On the following day distinct redness was visible, but no vesicular formation followed. After six days an inoculation of 1 c.c. of bouillon culture was made into the pleural cavity. Bapid breathing and rise of temperature followed, and the animal gradually sank with marasmus and dyspnoea, dying on the seventh day after inoculation. Post-mortem.—Slight pleurisy on the side of inoculation. Conges-