Physiological histology : methods and theory / by Gustav Mann.

  • Mann, Gustav, 1864-1921.
Date:
1902
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Licence: In copyright

Credit: Physiological histology : methods and theory / by Gustav Mann. Source: Wellcome Collection.

Provider: This material has been provided by King’s College London. The original may be consulted at King’s College London.

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    ADDITIONAL NOTE I. MICRO-ANATOMICAL REACTIONS. Elastic tissue. The first to describe a distinct stainiug method for elastic fibres with anilin red was v. Ebener^ and Martinotti^ has collected the older literature up to 1887. The dyes which have been chiefly used are Picric acid in the form of picro-carmin by Ranvier; Victoria blue in an alcoholic solution by Lustgarten^; Safranin (safranin 5 grms., absolute alcohol 100 cc, water 200 cc. for forty-eight hours), after fixation in 0-2 per cent, chromic acid by Martinotti; Fuchsin by Weigert, see below; Orcein by Tanzer and Unna, see below; Kreso-fachsin in an alcoholic solution by Eothig and Methyl-violet by myself in a watery solution after alcohol fixation. The latter stains elastic fibres deeply, but alcohol discharges the colour, and therefore the method of W. H. Welch (1876) should be employed. Welch dries sections partially with filter-paper (I use cigarette or thin toilet-paper), then covers them with xylene and alternately applies xylene and blotting-paper till the sections are quite clear. This is a very good method if we desire to preserve the actual staining efi'ect of watery stains. The best method for elastic fibres I consider to be Weigert's, as only elastic fibres are stained. The orcein methods are the next best, but the nuclei are also stained. Weigert's method for elastic fibres. The stain is prepared thus: Dissolve in a porcelain basin basic fuchsin or parafuchsin or new-fuchsin 2 grms. and resorcin 4 grms. in 200 cc. of water by boiling; add to the boiling mixture 25 cc. of the liquor ferri sesquichlor. Pharm. Germ. Ill, and continue to boil for some minutes.—I add sufficient of a saturated ferric chloride solution to precipitate the whole of the dye and to have a distinct yellow colouration of the supernatant solution. A black very sticky sediment forms in the basin; pour off as much of the solution as will flow off readily ; add 200 cc. of 95 per cent, alcohol and dissolve the sediment by boiling; filter while hot into, a bottle and add to the alcoholic solution, after it has '■ y. Ebener: Rollet's Uniers. a. d. Inst. f. Phys. u. Histol. in Gras (Leipzig, 1870), p. 32. * G. Martinotti : Zeitsch.f. wiss. Mikr. 4, 31 (1887). » Lustgarten : Med. Jahrb. d. Kaizerl. Konigl. Oes. d. Aerzle z. Wein, p. 285 (1886). * ROthig : Arch. /. mikr. Anat. 56, 354 (1900). This dyo stains elastic fibres blue, mucua, cartilage, and keratin red.