PCR cloning protocols / edited by Bing-Yuan Chen and Harry W. Janes.

Totowa, N.J. : Humana Press, [2002], ©2002.

xiv, 439 pages : illustrations ; 23 cm.

2nd ed.

Includes bibliographical references and index.

Polymerase chain reaction. Basic principles and routine practice / L.A. Kolmodin and D.E. Birch - - Computer programs for pcr primer design and analysis / B.Y. Chen, H.W. Janes and S. Chen - - Single-step pcr optimization using touchdown and stepdown pcr programming / K.H. Roux - - Xl pcr amplification of long targets from genomic DNA / L.A. Kolmodin - - Coupled one-step reverse transcription and polymerase chain reaction procedure for cloning large cdna fragments / J.T. Aatsinki - - Long distance reverse-transcription pcr / V. Thiel, J. Herold and S.G. Siddell - - Increasing pcr sensitivity for amplification from paraffin-embedded tissues / A. Akalu and J.K. Reichardt - - Gc-rich template amplification by inverse pcr. DNA polymerase and solvent effects / A. Moreau ... [et al.] - - Pcr procedure for the isolation of trinucleotide repeats / T. Tozaki - - Methylation-specific pcr / H. Ohashi - - Direct cloning of full-length cell differentially expressed genes by multiple rounds of subtractive hybridization based on long-distance pcr and magnetic beads / X. Huang, Z. Yuan and X. Cao - - Cloning pcr products. An overview / B. Guo and Y. Bi - - Using t4 DNA polymerase to generate clonable pcr products / K. Wang - - Enzyme-free cloning of pcr products and fusion protein expression / B.A. Neilan and D. Tillett - - Directional restriction site-free insertion of pcr products into vectors / G.J. Chen - - Autosticky pcr. Directional cloning of pcr products with performed 5' overhangs / J. Gal and M. Kalman - - A rapid and simple procedure for direct cloning of pcr products into baculoviruses / T.S. Gritsun, M.V. Mikhailov and E.A. Gould - - Pcr approaches to DNA mutagenesis and recombination. An overview / B. Shen - - In-frame cloning of synthetic genes using pcr inserts / J.C. Pierce - - Megaprimer pcr / S. Barik - - Pcr method for generating multiple mutations at adjacent sites / J. Adamec - - A fast polymerase chain reaction-mediated strategy for introducing repeat expansions into cag-repeat containing genes / F. Laccone - - Pcr screening in signature-tagged mutagenesis of essential genes / D.E. Lehoux and R.C. Levesque - - Staggered extension process (step) in vitro recombination / A.M. Aguinaldo and F. Arnold - - Random mutagenesis by whole-plasmid pcr amplification / D. Kim and F.P. Guengerich - - Pcr-based strategies to clone unknown DNA regions from known foreign integrants. An overview / E.K. Hui, P.C. Wang and S.J. Lo - - Long distance vectorette pcr (ldv pcr) / J.A. Fenton, G. Pratt and G.J. Morgan - - Nonspecific, nested suppression pcr method for isolation of unknown flanking DNA ("cold-start method") / M. Lardelli - - Inverse pcr. Cdna cloning / S.H. Huang - - Inverse pcr. Genomic DNA cloning / A.Y. Jong ... [et al.] - - Gene cloning and expression profiling by rapid amplification of gene inserts with universal vector primers / S.H. Huang, H.Y. Wu and A.Y. Jong - - The isolation of DNA sequences flanking tn5 transposon insertions by inverse pcr / V.J. Martin and W.W. Mohn - - Rapid amplification of genomic DNA sequences tagged by insertional mutagenesis / M. Celerin and K.T. Chun - - Isolation of large-terminal sequences of bac inserts based on double-restriction-enzyme digestion followed by anchored pcr / Z.N. Yang and T.E. Mirkov - - A "step down" pcr-based technique for walking into and the subsequent direct-sequence analysis of flanking genomic DNA / Z. Zhang and S.J. Gurr - - Use of pcr in library screening. An overview / J. Zhu - - Cloning of homologous genes by gene-capture pcr / R. Mastrangeli and S. Donini - - Rapid and nonradioactive screening of recombinant libraries by pcr / M.W. King - - Rapid cdna cloning by pcr screening (rc-pcr) / T. Takumi - - Generation and pcr screening of bacteriophage lambda sublibraries enriched for rare clones (the "sublibrary method") / M. Lardelli - - Pcr-based screening for bacterial artificial chromosome libraries / Y. Yasukochi - - A 384-well microtiter-plate-based template preparation and sequencing method / L. He and K. Wang - - A microtiter-plate-based high throughput pcr product purification method / R. Smith and K. Wang.

Performing and optimizing PCR -- Mutagenesis and recombination -- Cloning unknown neighboring DNA -- Library construction and screening.

Copy 1 Donor: Wellcome Trust Sanger Institute.