The nature of leucocytosis produced by nucleinic acid : a preliminary experimental study / by Delano Ames and A. A. Huntley.

  • Ames, Delano, 1906-1987.
Date:
1897
Catalogue details

Licence: Public Domain Mark

Credit: The nature of leucocytosis produced by nucleinic acid : a preliminary experimental study / by Delano Ames and A. A. Huntley. Source: Wellcome Collection.

Provider: This material has been provided by UCL Library Services. The original may be consulted at UCL (University College London)

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    2 c.c. of bacterial protein and immediately withdrew blood from the other ear, in which he found on count- ing the leucocytes only 3,300 as against 11,100 before the administration of the protein. As rapidly as pos- sible, counts were made from several internal vessels, the average of which was 21,500. From these figures he draws the conclusion that the decrease and there- fore also the subsequent increase, is only apparent, and that at first the leucocytes left the peripheral cir- culation for the central, and afterward the central for the peripheral. He evidently overlooked the fact, that had he estimated the total number of leucocytes before and after the injection he would have found that in the very short time that it took him to make the experiment there had been an actual increase in the whole number of 1,300 white cells per cubic mil- limeter, even though the cells at the time of the count were unevenly distributed. From all the facts that we have cited it would appear that we are justified in looking on the change in the circulation that follows an injection of nuclein as a leucocytosis in which there is an increase in the total number of white corpuscles. The experiments that we made were all upon medium-sized dogs, and while we will not detail each one we will give the steps pursued and a few of the results which are typical of all, since with very slight variations practically the same results were obtained in all cases. Before putting the dog under ether a count was made of the leucocytes in the peripheral circulation. Ether was then administered and the same was done for the peripheral and central circula- tions. We made the count of the peripheral circula- tion before the administration of ether, because we found that the ether alone produced some increase in the number of leucocytes, the amount seeming to depend on the rapidity or slowness with which the animal succumbed to the anesthetic; a greater change was noted in those dogs that struggled the most and required the most ether. After making the counts