Remarks on a new method (interlamellar films) of studying the development of micro-organisms and the mutability of their characters and properties / by Sheridan Délepine.

  • Delépine, Sheridan, 1855-1921.
Date:
[1891]
Catalogue details

Licence: Public Domain Mark

Credit: Remarks on a new method (interlamellar films) of studying the development of micro-organisms and the mutability of their characters and properties / by Sheridan Délepine. Source: Wellcome Collection.

Provider: This material has been provided by King’s College London. The original may be consulted at King’s College London.

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    viously inoculated). Then the cover is placed over the drop. It should be well supported by the three drops of sealing-wax, and should not at this stage flatten much the drop under- neath. A heated rod is then applied successively over the three drops of sealing-wax until the inoculated lluid has spread evenly over a certain surface under the central parts of the cover; the preparation is then ready for the incu- bator. It has, however, to be kept in mind that, owing to the free access air has to the surface of the inoculated lluid, it is necessary to keep the preparation in a moist chamber. The extremely small size of these inoculated slides allows of a large number being packed in an extremely small space. Before incubating the preparation it is necessary to select out of the micro-organism3 which have been sown into the fluid one or several, the position and relation of which are carefully noted. For this purpose divided cover-glasses or slides, or, as I prefer, a finder, can be used. I have in this way followed the development of bacteria and spores of pathogenic Pyrenomycetes for days and weeks. I have devised many improvements for regulating the thickness of the enclosed film and making its thickness perfectly even, but these are not necessary to the success of the method, and their description would obscure its main object. Now it will appear to many that this is nothing else than a drop cultivation, and to others that it is a film or a plate cultivation. It is all this, but I claim that it is something more; for by using an interlamellar film,” as I feel in- clined to call it, the free surface of the medium is limited to the space existing between the two glass lamellm used, whilst in ordinary plate, film, or drop cultivations the surface in contact with the air is very extensive; by the interlamellar method a side view, so to speak, of the cultivation is obtained ; by the drop method a surface view. By the interlamellar method organisms placed at various distances from the free surface of the medium can be