Chemical examination of the roots of Phaseolus multiflorus / by Frederick B. Power and Arthur H. Salway.

  • Power, Frederick B. (Frederick Belding), 1853-1927.
Date:
[1913]
Catalogue details

Licence: In copyright

Credit: Chemical examination of the roots of Phaseolus multiflorus / by Frederick B. Power and Arthur H. Salway. Source: Wellcome Collection.

Provider: This material has been provided by The Royal College of Surgeons of England. The original may be consulted at The Royal College of Surgeons of England.

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    a considerable depression of the melting point ensued. On the other hand, it agreed closely in its characters with the furan- ,CH=CH /3-carboxylic acid, 0 which n\ch=ccooh, was recently isolated for the first time by Rogerson2 from the bark of Euonymus atropurpureus, Jacquin, this acid having been found to melt at 121-122°, and to yield a methyl ester possessing an odour very similar to that of methyl benzoate. When a little of the above-described substance was mixed with furan-/3- carboxylic acid, no depression of the melting-point was ob- served. No doubt respecting the identity of the two sub- stances could, therefore, be entertained. The ethereal liquid, which had been shaken with aqueous ammonium carbonate, as above described, was next extracted successively with aqueous sodium carbonate and sodium hydroxide, but nothing definite was isolated from these ex- tracts nor from the ethereal liquid remaining after this treat- ment. The aqueous liquid which had been extracted with ether, was subsequently extracted several times with amyl alcohol, the latter extracts being then united, washed with a little water, and evaporated to dryness under diminished pressure. A small quantity (about 5 grammes) of a dark-coloured, viscid residue was thus obtained, which was glucosidic in char- acter, but nothing definite could be isolated from it. The aqueous liquid which had been extracted with both ether and amyl alcohol, as above described, had a dark-brown colour, and it was, therefore, treated with a slight excess of basic lead acetate. An abundant light-brown precipitate was thus produced, which was collected, suspended in water, decomposed by hydrogen sulphide, and the mixture filtered! The filtered liquid gave on evaporation an amorphous pro- duct, which was of an indefinite character, and appeared to contain no tannin. The filtrate from the basic lead acetate precipitate was deprived of lead by means of hydrogen sul- phide, and concentrated under diminished pressure to the consistency of a syrup. This evidently contained a consider- able proportion of sugar, since it readily reduced Fehlin^’s solution, and from a small portion of the liquid d-phenvl- gluoosazone (m.p. 212°) was prepared. Or, heating a little ot the liquid with an alkali hydroxide it developed ammonia. Isolation of Allantoin, C4H(103N,1. 1 he syrupy, aqueous liquid, which had been purified by means of basic lead acetate, as above described, was found to yield a precipitate with mercuric nitrate in the presence of dilute nitric acid. The. entire remaining portion of the aqueous liquid was, therefore, treated with this reagent, the precipitate collected, well washed with hot water, then sus- pended in water, and decomposed by hydrogen sulphide. The nitrate, after removing the excess of hydrogen sulphide, was 2 Journ, Cheni. ,Soc., 1912, 101,1041.